High-pressure carbon monoxide and oxygen mixture is effective for lung preservation

( 最大 2000 件 )
URI http://shark.lib.kagawa-u.ac.jp/kuir/metadata/28832
タイトル
High-pressure carbon monoxide and oxygen mixture is effective for lung preservation
ファイル
内容記述

Abstract

(1) Background: Heme oxygenase-1 (HO-1) degrades heme and generates carbon monoxide (CO), producing various anti-inflammatory, anti-oxidative, and anti-apoptotic effects. This study aimed to confirm the effects of CO on the ischemia-reperfusion injury (IRI) of donor lungs using a high-pressure gas (HPG) preservation method. (2) Methods: Donor rat and canine lungs were preserved in a chamber filled with CO (1.5 atm) and oxygen (O2; 2 atm) and were ventilated with either CO and O2 mixture (CO/O2 group) or air (air group) immediately before storage. Rat lungs were subjected to heterotopic cervical transplantation and evaluated after reperfusion, whereas canine lungs were subjected to allogeneic transplantation and evaluated. (3) Results: Alveolar hemorrhage in the CO/O2 group was significantly milder than that in the air group. mRNA expression levels of HO-1 remained unchanged in both the groups; however, inflammatory mediator levels were significantly lower in the CO/O2 group than in the air group. The oxygenation of graft lungs was comparable between the two groups, but lactic acid level tended to be higher in the air group. (4) Conclusions: The HO-1/CO system in the HPG preservation method is effective in suppressing IRI and preserving donor lungs.

KEYWORDS:

carbon monoxide; high-pressure gas; ischemia–reperfusion injury; lung preservation

(医博甲728)

著者
著者 藤原 敦史
著者(ヨミ) フジワラ アツシ
著者(別表記) Fujiwara Atsushi
掲載誌
International Journal of Molecular Sciences
掲載誌(別表記)
Int J Mol Sci.
20
11
出版者
MDPI
出版年月日
2019-06-03
ISSN
1422-0067
NCID
AA12038549
PMID
31163581
DOI
10.3390/ijms20112719
資料タイプ
学位論文
言語
英語
関連情報
PMCID: PMC6600409
関連情報URL
https://doi.org/10.3390/ijms20112719
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6600409/
権利関係
Copyright © 2019 by the authors.
"This is the Publisher's Version of the following article: High-pressure carbon monoxide and oxygen mixture is effective for lung preservation; International Journal of Molecular Sciences; Volume 20 Issue 11 (2019) doi: 10.3390/ijms20112719, which has been published in final form at https://doi.org/10.3390/ijms20112719 . "
Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) International license 4.0 (http://creativecommons.org/licenses/by/4.0/).
doi: https://doi.org/10.3390/ijms20112719
博士論文(全文を含む)
学位授与番号
博甲第728号
学位授与年月日
2019-12-25
学位名
博士(医学)
学位授与機関
香川大学
区分
香川大学
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