Light exercise without lactate elevation induces ischemic tolerance through the modulation of microRNA in the gerbil hippocampus

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URI http://shark.lib.kagawa-u.ac.jp/kuir/metadata/28998
Title
Light exercise without lactate elevation induces ischemic tolerance through the modulation of microRNA in the gerbil hippocampus
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Description

Abstract

Previously we studied the possible neuroprotective effects of ischemia-resistant exercise in a gerbil model of transient whole-brain ischemia and evaluated the histology, expression of specific proteins, and brain function under different conditions. The present study investigated the neuroprotective effects of light exercise, without lactate elevation, in a gerbil model of ischemia/reperfusion injury. Transient whole-brain ischemia was induced by occlusion of the bilateral common carotid arteries for 5 min. A group of animals was subjected to treadmill exercise before ischemia induction. Hippocampal neuronal damage and miRNA expression, as well as behavioral deficits and plasma lactate levels, were evaluated. Light exercise suppressed hippocampal neuron loss and preserved short-term memory. Moreover, 14 miRNAs (mmu-miR-211-3p, -327, -451b, -711, -3070-3p, -3070-2-3p, -3097-5p, -3620-5p, -6240, -6916-5p, -6944-5p, 7083-5p, -7085-5p, and -7674-5p) were upregulated and 6 miRNAs (mmu-miR-148b-3p, -152-3p, -181c-5p, -299b-5p, -455-3p, and -664-3p) were downregulated due to ischemia. However, the expression of these miRNAs remained unchanged when animals performed light exercise before the ischemic event. Differentially expressed miRNAs regulate multiple biological processes such as inflammation, metabolism, and cell death. These findings suggest that light exercise reduces neuronal death and behavioral deficits after transient ischemia by regulating hippocampal miRNAs.

Keywords:

Hippocampus; Ischemic tolerance exercise; Lactate; MicroRNA; Reperfusion; Short-term memory;

(医博甲748)

Author
著者 高田 忠幸
著者(ヨミ) タカタ タダユキ
著者(別表記) Takata Tadayuki
Publication Title
Brain Research
Publication Title Alternative
Brain Res.
Volume
1732
Publisher
Elsevier
Published Date
2020-04-01
ISSN
0006-8993
NCID
AA10800122
PMID
32035888
DOI
10.1016/j.brainres.2020.146710
Resource Type
Thesis or Dissertation
Language
eng
Rights
Copyright © 2020 Elsevier B.V.
"This is the Publisher's Version of the following article: Light exercise without lactate elevation induces ischemic tolerance through the modulation of microRNA in the gerbil hippocampus; Brain Research; Volume 1732 Article 146710(2020); doi: 10.1016/j.brainres.2020.146710 , which has been published in final form at https://doi.org/10.1016/j.brainres.2020.146710 . "
この博士論文の本文については、次のエルゼビアの著作権ポリシーの規定により公開しています。「博士論文の場合は、エンバーゴ期間に関係なく機関リポジトリに出版社版を公開することができます。」 The text of this doctoral dissertation is published according to the following Elsevier copyright policy. "In the case of doctoral dissertations, publishers can be published in institutional repositories regardless of the Embargo period."
Text Version
ETD
Grant ID
博甲第748号
Grant Date
2020-06-25
Degree Name
博士(医学)
Grantor
香川大学
Set
香川大学
Copyright (C) 2009 Kagawa University All rights reserved.