Light exercise without lactate elevation induces ischemic tolerance through the modulation of microRNA in the gerbil hippocampus

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URI http://shark.lib.kagawa-u.ac.jp/kuir/metadata/28998
タイトル
Light exercise without lactate elevation induces ischemic tolerance through the modulation of microRNA in the gerbil hippocampus
ファイル
内容記述

Abstract

Previously we studied the possible neuroprotective effects of ischemia-resistant exercise in a gerbil model of transient whole-brain ischemia and evaluated the histology, expression of specific proteins, and brain function under different conditions. The present study investigated the neuroprotective effects of light exercise, without lactate elevation, in a gerbil model of ischemia/reperfusion injury. Transient whole-brain ischemia was induced by occlusion of the bilateral common carotid arteries for 5 min. A group of animals was subjected to treadmill exercise before ischemia induction. Hippocampal neuronal damage and miRNA expression, as well as behavioral deficits and plasma lactate levels, were evaluated. Light exercise suppressed hippocampal neuron loss and preserved short-term memory. Moreover, 14 miRNAs (mmu-miR-211-3p, -327, -451b, -711, -3070-3p, -3070-2-3p, -3097-5p, -3620-5p, -6240, -6916-5p, -6944-5p, 7083-5p, -7085-5p, and -7674-5p) were upregulated and 6 miRNAs (mmu-miR-148b-3p, -152-3p, -181c-5p, -299b-5p, -455-3p, and -664-3p) were downregulated due to ischemia. However, the expression of these miRNAs remained unchanged when animals performed light exercise before the ischemic event. Differentially expressed miRNAs regulate multiple biological processes such as inflammation, metabolism, and cell death. These findings suggest that light exercise reduces neuronal death and behavioral deficits after transient ischemia by regulating hippocampal miRNAs.

Keywords:

Hippocampus; Ischemic tolerance exercise; Lactate; MicroRNA; Reperfusion; Short-term memory;

(医博甲748)

著者
著者 高田 忠幸
著者(ヨミ) タカタ タダユキ
著者(別表記) Takata Tadayuki
掲載誌
Brain Research
掲載誌(別表記)
Brain Res.
1732
出版者
Elsevier
出版年月日
2020-04-01
ISSN
0006-8993
NCID
AA10800122
PMID
32035888
DOI
10.1016/j.brainres.2020.146710
資料タイプ
学位論文
言語
英語
権利関係
Copyright © 2020 Elsevier B.V.
"This is the Publisher's Version of the following article: Light exercise without lactate elevation induces ischemic tolerance through the modulation of microRNA in the gerbil hippocampus; Brain Research; Volume 1732 Article 146710(2020); doi: 10.1016/j.brainres.2020.146710 , which has been published in final form at https://doi.org/10.1016/j.brainres.2020.146710 . "
この博士論文の本文については、次のエルゼビアの著作権ポリシーの規定により公開しています。「博士論文の場合は、エンバーゴ期間に関係なく機関リポジトリに出版社版を公開することができます。」 The text of this doctoral dissertation is published according to the following Elsevier copyright policy. "In the case of doctoral dissertations, publishers can be published in institutional repositories regardless of the Embargo period."
博士論文(全文を含む)
学位授与番号
博甲第748号
学位授与年月日
2020-06-25
学位名
博士(医学)
学位授与機関
香川大学
区分
香川大学
Copyright (C) 2009 Kagawa University All rights reserved.