The Effect of Gemcitabine on Cell Cycle Arrest and microRNA Signatures in Pancreatic Cancer Cells
URI | http://shark.lib.kagawa-u.ac.jp/kuir/metadata/29142 | ||||||
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Title |
The Effect of Gemcitabine on Cell Cycle Arrest and microRNA Signatures in Pancreatic Cancer Cells
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Description |
Abstract Background/aim: Gemcitabine, an inhibitor of DNA synthesis, is the gold standard chemotherapeutic agent for pancreatic ductal adenocarcinoma (PDAC). MicroRNAs (miRNAs) play critical roles in cancers, including PDAC. However, less is known about the effect of gemcitabine on PDAC cells and miRNA expression in PDAC. We evaluated the effect of gemcitabine on the cell cycle of PDAC cells in vitro and in vivo and on the miRNA expression profile. Materials and methods: Effects of gemcitabine on PK-1 and PK-9 cell growth were evaluated using a cell counting kit-8 assay. Xenografted mouse models were used to assess gemcitabine effects in vivo. Results: Gemcitabine inhibited the proliferation and tumour growth of PK-1 cells, and induced S phase cell cycle arrest. Numerous miRNAs were altered upon gemcitabine treatment of PK-1 cells and xenograft models. Conclusion: Altered miRNAs may serve as potential therapeutic targets for improving the efficacy of gemcitabine in PDAC. Keywords: Pancreatic neoplasm; cell cycle; cyclin; gemcitabine; microRNA (医博甲759) |
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Author |
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Publication Title |
In Vivo
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Publication Title Alternative |
In Vivo.
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Volume |
34
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Issue |
6
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Start Page |
3195
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End Page |
3203
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Publisher |
International Institute of Anticancer Research
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Published Date |
2020
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ISSN |
0258-851X
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NCID |
AA10714348
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PMID |
33144424
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DOI |
10.21873/invivo.12155
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Resource Type |
Thesis or Dissertation
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Language |
eng
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Relation |
PMCID:PMC781167
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Resource URL |
http://www.ncbi.nlm.nih.gov/pmc/articles/pmc7811671/
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Rights |
Copyright© 2020 International Institute of Anticancer Research
This is the Publisher's Version of the following article: The Effect of Gemcitabine on Cell Cycle Arrest and microRNA Signatures in Pancreatic Cancer Cells; In Vivo; Volume 34 Issue 6 (2020) Pages: 3195-3203; doi: 10.21873/invivo.12155 , which has been published in final form at https://doi.org/10.21873/invivo.12155 .
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Text Version |
ETD
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Grant ID |
博甲第759号
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Grant Date |
2020-12-24
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Degree Name |
博士(医学)
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Grantor |
香川大学
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Set |
香川大学
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