The Effect of Gemcitabine on Cell Cycle Arrest and microRNA Signatures in Pancreatic Cancer Cells

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URI http://shark.lib.kagawa-u.ac.jp/kuir/metadata/29142
Title
The Effect of Gemcitabine on Cell Cycle Arrest and microRNA Signatures in Pancreatic Cancer Cells
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Description

Abstract

Background/aim: Gemcitabine, an inhibitor of DNA synthesis, is the gold standard chemotherapeutic agent for pancreatic ductal adenocarcinoma (PDAC). MicroRNAs (miRNAs) play critical roles in cancers, including PDAC. However, less is known about the effect of gemcitabine on PDAC cells and miRNA expression in PDAC. We evaluated the effect of gemcitabine on the cell cycle of PDAC cells in vitro and in vivo and on the miRNA expression profile.

Materials and methods: Effects of gemcitabine on PK-1 and PK-9 cell growth were evaluated using a cell counting kit-8 assay. Xenografted mouse models were used to assess gemcitabine effects in vivo.

Results: Gemcitabine inhibited the proliferation and tumour growth of PK-1 cells, and induced S phase cell cycle arrest. Numerous miRNAs were altered upon gemcitabine treatment of PK-1 cells and xenograft models.

Conclusion: Altered miRNAs may serve as potential therapeutic targets for improving the efficacy of gemcitabine in PDAC.

Keywords:

Pancreatic neoplasm; cell cycle; cyclin; gemcitabine; microRNA

(医博甲759)

Author
著者 波間 大輔
著者(ヨミ) ナミマ ダイスケ
著者(別表記) Daisuke Namima
Publication Title
In Vivo
Publication Title Alternative
In Vivo.
Volume
34
Issue
6
Start Page
3195
End Page
3203
Publisher
International Institute of Anticancer Research
Published Date
2020
ISSN
0258-851X
NCID
AA10714348
PMID
33144424
DOI
10.21873/invivo.12155
Resource Type
Thesis or Dissertation
Language
eng
Relation
PMCID:PMC781167
Resource URL
http://www.ncbi.nlm.nih.gov/pmc/articles/pmc7811671/
Rights
Copyright© 2020 International Institute of Anticancer Research
This is the Publisher's Version of the following article: The Effect of Gemcitabine on Cell Cycle Arrest and microRNA Signatures in Pancreatic Cancer Cells; In Vivo; Volume 34 Issue 6 (2020) Pages: 3195-3203; doi: 10.21873/invivo.12155 , which has been published in final form at https://doi.org/10.21873/invivo.12155 .
Text Version
ETD
Grant ID
博甲第759号
Grant Date
2020-12-24
Degree Name
博士(医学)
Grantor
香川大学
Set
香川大学
Copyright (C) 2009 Kagawa University All rights reserved.