The Effect of Gemcitabine on Cell Cycle Arrest and microRNA Signatures in Pancreatic Cancer Cells

( 最大 2000 件 )
URI http://shark.lib.kagawa-u.ac.jp/kuir/metadata/29142
タイトル
The Effect of Gemcitabine on Cell Cycle Arrest and microRNA Signatures in Pancreatic Cancer Cells
ファイル
内容記述

Abstract

Background/aim: Gemcitabine, an inhibitor of DNA synthesis, is the gold standard chemotherapeutic agent for pancreatic ductal adenocarcinoma (PDAC). MicroRNAs (miRNAs) play critical roles in cancers, including PDAC. However, less is known about the effect of gemcitabine on PDAC cells and miRNA expression in PDAC. We evaluated the effect of gemcitabine on the cell cycle of PDAC cells in vitro and in vivo and on the miRNA expression profile.

Materials and methods: Effects of gemcitabine on PK-1 and PK-9 cell growth were evaluated using a cell counting kit-8 assay. Xenografted mouse models were used to assess gemcitabine effects in vivo.

Results: Gemcitabine inhibited the proliferation and tumour growth of PK-1 cells, and induced S phase cell cycle arrest. Numerous miRNAs were altered upon gemcitabine treatment of PK-1 cells and xenograft models.

Conclusion: Altered miRNAs may serve as potential therapeutic targets for improving the efficacy of gemcitabine in PDAC.

Keywords:

Pancreatic neoplasm; cell cycle; cyclin; gemcitabine; microRNA

(医博甲759)

著者
著者 波間 大輔
著者(ヨミ) ナミマ ダイスケ
著者(別表記) Daisuke Namima
掲載誌
In Vivo
掲載誌(別表記)
In Vivo.
34
6
開始ページ
3195
終了ページ
3203
出版者
International Institute of Anticancer Research
出版年月日
2020
ISSN
0258-851X
NCID
AA10714348
PMID
33144424
DOI
10.21873/invivo.12155
資料タイプ
学位論文
言語
英語
関連情報
PMCID:PMC781167
関連情報URL
http://www.ncbi.nlm.nih.gov/pmc/articles/pmc7811671/
権利関係
Copyright© 2020 International Institute of Anticancer Research
This is the Publisher's Version of the following article: The Effect of Gemcitabine on Cell Cycle Arrest and microRNA Signatures in Pancreatic Cancer Cells; In Vivo; Volume 34 Issue 6 (2020) Pages: 3195-3203; doi: 10.21873/invivo.12155 , which has been published in final form at https://doi.org/10.21873/invivo.12155 .
博士論文(全文を含む)
学位授与番号
博甲第759号
学位授与年月日
2020-12-24
学位名
博士(医学)
学位授与機関
香川大学
区分
香川大学
Copyright (C) 2009 Kagawa University All rights reserved.