Effects of VEGF on Prefabricated Vascularized Bone Allografts in Rats

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URI http://shark.lib.kagawa-u.ac.jp/kuir/metadata/29145
Title
Effects of VEGF on Prefabricated Vascularized Bone Allografts in Rats
File
Description

Abstract

Background: Massive bone defects after wide resection of malignant bone tumors or a serious injury require treatment using vascularized bone grafts. Although cadaveric bone allografts combined with vascularized bone autografts are currently thought to be ideal in terms of size and durability, this treatment requires the scarification of healthy bone tissue. In a previous study, we attempted to improve this situation by prefabricating a vascularized bone allograft in recipient rats. In this study, we added vascular endothelial growth factor (VEGF)-containing hydroxyapatite/collagen composite (HAp/Col) to a prefabricated vascularized bone allograft to stimulate angiogenesis, which is known to be important for bone formation.

Materials and methods: Sprague Dawley rats (n = 50) were used as donors and Wistar rats (n = 50) as recipients. All rats were 9 weeks old. The recipient rats were divided into five groups according to the use of vascular bundles, HAp/Col, and an additive substance (VEGF). The bone allografts collected from the donors were transplanted into the thigh region of the recipients, and a saphenous vein and 10 μg HAp/Col with VEGF were inserted into the bone allografts through the slit. After 4 weeks, the transplanted bone allografts were harvested, and histologic and genetic evaluations were performed in relation to bone formation and resorption.

Results: The results showed that, compared with the control group, the implantation of the vascular bundles and VEGF-containing HAp/Col significantly stimulated angiogenesis and bone formation in the rats with the bone allografts. However, histological and genetic evaluations of bone resorption revealed that resorption was not observed in any group.

Conclusion: These results suggest that VEGF-containing HAp/Col effectively stimulates angiogenesis and bone formation, but not bone resorption, in prefabricated vascularized bone allografts. This method could therefore become a useful tool for treating large bone defects.

Keywords:

vascular endothelial growth factor; basic fibroblast growth factor; allograft

(医博甲762)

Author
著者 飛梅 祥子
著者(ヨミ) トビウメ サチコ
著者(別表記) Sachiko Tobiume
Publication Title
Journal of Reconstructive Microsurgery
Publication Title Alternative
J Reconstr Microsurg.
Publisher
American Society for Reconstructive Microsurgery
Thieme Medical Publishers
Published Date
2020-10-14
ISSN
0743-684X
NCID
AA10640401
PMID
33058099
DOI
10.1055/s-0040-1718394
Resource Type
Thesis or Dissertation
Language
eng
Rights
Copyright © 2020 Thieme.
This is the Accepted Version of the following article: Effects of VEGF on Prefabricated Vascularized Bone Allografts in Rats; Journal of Reconstructive Microsurgery; Online ahead of print (2020) doi: 10.1055/s-0040-1718394, which has been published in final form at https://doi.org/10.1055/s-0040-1718394 .
Text Version
none
Grant ID
博甲第762号
Grant Date
2020-12-24
Degree Name
博士(医学)
Grantor
香川大学
Set
香川大学
Copyright (C) 2009 Kagawa University All rights reserved.